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ALAS Berlese Collections, 2001 Expeditions
Berlese samples were not precisely georeferenced. Sample sites were subjectively chosen, stratified by the desired microhabitats. All samples were taken within 100m of the refuge building. Samples were approximately 1.5l in volume. Samples were either "hand" samples or "ring" samples. For hand samples, substrate was gathered by hand. For ring samples, the sample was taken by quickly placing a 14.5 cm inside diameter, 10cm deep PVC ring on top of the leaf litter, driving it into the ground, dislodging it, and placing the material in a bag. Samples were all stored in cloth bags, packed into backpacks, hiked out of the site, transported to the lab at La Selva, and suspended in Berlese funnels. All samples were placed in Berlese funnels within 24 hours of being obtained from the field. Collapsible cloth funnels were used. The sample bed was a 35 x 35cm piece of 6.6mm mesh hardware cloth, overlain with several layers of cheesecloth. The soil sample was broken apart and spread out on the cheesecloth. An incandescent light bulb was placed 15-20cm above the sample bed and the bulb and the sample covered with a pyramidal aluminum-foil shield. The first 36 samples were left under a 25 watt bulb for two days, then under a 50 watt bulb for three days. All subsequent samples were left under a 50 watt bulb for five days. Arthropods were collected in a Whirlpac bag filled with 95% ethanol suspended from the bottom of the funnel.
Microhabitat codes for the samples, incorporated into the collection code (see below), are as follows:
AS: ring sample, leaf litter and mineral soil
AH: ring sample, deep leaf litter
AM: ring sample, rotten wood
BS: hand sample, leaf litter and mineral soil
BH: hand sample, leaf litter
BM: hand sample, rotten wood
BF: hand sample, fungi
BE: hand sample, epiphytes
BV: hand sample, various substrates
The data fields, in order, are:
Collection code: a unique code for a collection. The "11" indicates that it is from the 1070m site, the "B" indicates it is a Berlese sample, the following two letters are the microhabitat code (see above), and the final three-digit number is a serial number beginning with 001 for the first Berlese sample and ending with 120 for the last Berlese sample.
Collector: ALAS refers to the ALAS staff at the site (Danilo Brenes, Maylin Paniagua, Ronald Vargas).
Collection date: in format day-month-year.
Source: further notes on substrate.
11/B/AS/001 ALAS 8-Feb-2001 a orrilla de quebrada
11/B/AS/002 ALAS 8-Feb-2001 detras de tronco
11/B/AS/003 ALAS 8-Feb-2001 Hojarasca
11/B/AS/004 ALAS 8-Feb-2001 Hojarasca
11/B/AS/005 ALAS 8-Feb-2001 al lado de tronco caido
11/B/AS/006 ALAS 8-Feb-2001 isla de quebrada
11/B/AS/007 ALAS 8-Feb-2001 cerca de tronco recien cortado
11/B/AS/008 ALAS 8-Feb-2001 cerca de quebrada
11/B/AS/009 ALAS 8-Feb-2001 cerca de quebrada
11/B/AS/010 ALAS 8-Feb-2001 cerca de quebrada
11/B/AS/011 ALAS 8-Feb-2001 al pie de un tronco cerca de quebrada
11/B/AS/012 ALAS 8-Feb-2001 cerca de quebrada
11/B/BH/013 V. Behan-Pelletier 9-Feb-2001 under tree fern, near emergent tree
11/B/BE/014 V. Behan-Pelletier 9-Feb-2001 soil of suspended soil mat
11/B/BH/015 V. Behan-Pelletier 9-Feb-2001 mixed primary forest litter
11/B/BS/016 V. Behan-Pelletier 9-Feb-2001 litter under tree fern
11/B/BE/017 V. Behan-Pelletier 9-Feb-2001 suspended soil and epiphytes
11/B/BM/018 V. Behan-Pelletier 9-Feb-2001 decaying wood from log lying on litter
11/B/BE/019 V. Behan-Pelletier 9-Feb-2001 epiphytic ferns and suspended soil
11/B/BH/020 V. Behan-Pelletier 9-Feb-2001 mixed litter in primary forest, nr Inga
11/B/BM/021 V. Behan-Pelletier 9-Feb-2001 decaying log by stream
11/B/BS/022 V. Behan-Pelletier 9-Feb-2001 Mixed primary forest litter & soil
11/B/BE/023 V. Behan-Pelletier 9-Feb-2001 Epiphytic ferns & orchids
11/B/BS/024 ALAS 9-Feb-2001 litter & soil near giant Inga sp.
11/B/BV/025 ALAS 14-Feb-2001 A lo largo quebrada derrumbe.
11/B/BV/026 ALAS 14-Feb-2001 A lo largo quebrada derrumbe.
11/B/BV/027 ALAS 14-Feb-2001 A lo largo quebrada derrumbe.
11/B/BS/028 ALAS 14-Feb-2001 A lo largo quebrada derrumbe.
11/B/BE/029 ALAS 14-Feb-2001 A lo largo quebrada derrumbe.
11/B/BV/030 ALAS 14-Feb-2001 A lo largo quebrada derrumbe.
11/B/AS/031 ALAS 14-Feb-2001 A lo largo del sendero hacia abajo.
11/B/BS/032 ALAS 14-Feb-2001 A lo largo del sendero hacia abajo.
11/B/AM/033 ALAS 14-Feb-2001 A lo largo del sendero hacia abajo.
11/B/BV/034 ALAS 14-Feb-2001 A lo largo del sendero hacia abajo.
11/B/AS/035 ALAS 14-Feb-2001 A lo largo del sendero hacia abajo.
11/B/AS/036 ALAS 14-Feb-2001 A lo largo del sendero hacia abajo.
11/B/BM/037 ALAS 20-Feb-2001 madera muerta caida cerca quebrada
11/B/BV/038 ALAS 20-Feb-2001 tierra gambas de Inga.
11/B/AS/039 ALAS 20-Feb-2001 en gambas de Inga.
11/B/AS/040 ALAS 20-Feb-2001 cerca M/06
11/B/BV/041 ALAS 20-Feb-2001 suelo suspendido
11/B/BV/042 ALAS 20-Feb-2001 cosas suspendidas
11/B/AM/043 ALAS 20-Feb-2001 a lo largo camino M/06 y M/10.
11/B/BM/044 ALAS 20-Feb-2001 Arbol muerto en pie.
11/B/BE/045 ALAS 20-Feb-2001 a lo largo camino M/06 y M/10.
11/B/BM/046 ALAS 20-Feb-2001 Raices Euterpe muerto.
11/B/BH/047 ALAS 20-Feb-2001 a lo largo camino M/06 y M/10.
11/B/AS/048 ALAS 20-Feb-2001 a lo largo camino M/06 y M/10.
11/B/BV/049 ALAS 9-Mar-2001 A lo largo del sendero arriba musgo
11/B/BV/050 ALAS 9-Mar-2001 musgo y hojarasca
11/B/BE/051 ALAS 9-Mar-2001 epifitas
11/B/BM/052 ALAS 9-Mar-2001 A lo largo del sendero hacia arriba.
11/B/BV/053 ALAS 9-Mar-2001 suelo y hojarasca suspendido en helecho
11/B/BH/054 ALAS 9-Mar-2001 A lo largo del sendero hacia arriba.
11/B/AH/055 ALAS 9-Mar-2001 detras del refugio
11/B/AS/056 ALAS 9-Mar-2001 detras del refugio (suelo bajo tronco)
11/B/AS/057 ALAS 9-Mar-2001 detras del refugio
11/B/AM/058 ALAS 9-Mar-2001 detras del refugio
11/B/AS/059 ALAS 9-Mar-2001 detra del refugio (raices)
11/B/BV/060 ALAS 9-Mar-2001 suelo suspendido en tronco muerto
11/B/AS/061 ALAS 15-Mar-2001 suelo
11/B/BM/062 ALAS 15-Mar-2001 madera podrida
11/B/BE/063 ALAS 15-Mar-2001 epifitas
11/B/BS/064 ALAS 15-Mar-2001 suelo
11/B/BV/065 ALAS 15-Mar-2001 musgo suspendido
11/B/AM/066 ALAS 15-Mar-2001 madera podrida
11/B/BV/067 ALAS 15-Mar-2001 hojarasca suspendida en helecho
11/B/AH/068 ALAS 15-Mar-2001 hojarasca
11/B/BV/069 ALAS 15-Mar-2001 musgo suspendido
11/B/AH/070 ALAS 15-Mar-2001 hojarasca
11/B/AS/071 ALAS 15-Mar-2001 suelo
11/B/BV/072 ALAS 15-Mar-2001 suelo tronco hueco
11/B/BV/073 ALAS 21-Mar-2001 raices helecho vivo
11/B/AS/074 ALAS 21-Mar-2001 suelo
11/B/BM/075 ALAS 21-Mar-2001 madera podrida
11/B/BS/076 ALAS 21-Mar-2001 suelo suspendido
11/B/BH/077 ALAS 21-Mar-2001 hojarasca
11/B/AM/078 ALAS 21-Mar-2001 madera podrida
11/B/BE/079 ALAS 21-Mar-2001 epifitas
11/B/BE/080 ALAS 21-Mar-2001 epifitas
11/B/AS/081 ALAS 21-Mar-2001 suelo
11/B/BM/082 ALAS 21-Mar-2001 madera podrida suspendida
11/B/AM/083 ALAS 21-Mar-2001 madera podrida
11/B/AH/084 ALAS 21-Mar-2001 hojarasca
11/B/BS/085 ALAS 9-Apr-2001 suelo
11/B/BV/086 ALAS 9-Apr-2001 suelo suspendido
11/B/BE/087 ALAS 9-Apr-2001 epifitas
11/B/BH/088 ALAS 9-Apr-2001 hojarasca
11/B/BM/089 ALAS 9-Apr-2001 tronco podrido
11/B/BM/090 ALAS 9-Apr-2001 tronco podrido hojarasca
11/B/BE/091 ALAS 9-Apr-2001 epifitas
11/B/BM/092 ALAS 9-Apr-2001 tronco podrido
11/B/BV/093 ALAS 9-Apr-2001 varios
11/B/AM/094 ALAS 9-Apr-2001 madera podrida
11/B/BV/095 ALAS 9-Apr-2001 raices helecho
11/B/AS/096 ALAS 9-Apr-2001 suelo
11/B/AS/097 ALAS 15-Apr-2001 suelo
11/B/BV/098 ALAS 15-Apr-2001 hojarasca suspendida
11/B/AS/099 ALAS 15-Apr-2001 suelo
11/B/AH/100 ALAS 15-Apr-2001 hojarasca
11/B/BV/101 ALAS 15-Apr-2001 hojarasca suspendida
11/B/BE/102 ALAS 15-Apr-2001 epifitas
11/B/AM/103 ALAS 15-Apr-2001 madera podrida
11/B/AH/104 ALAS 15-Apr-2001 hojarasca
11/B/BE/105 ALAS 15-Apr-2001 epifitas
11/B/BV/106 ALAS 15-Apr-2001 hojarasca suspendida
11/B/BV/107 ALAS 15-Apr-2001 aserrin viejo donde cortaron un tronco
11/B/AS/108 ALAS 15-Apr-2001 suelo
11/B/BV/109 ALAS 21-Apr-2001 material suspendido
11/B/BM/110 ALAS 21-Apr-2001 tronco en pie muerto
11/B/AS/111 ALAS 21-Apr-2001 suelo
11/B/AH/112 ALAS 21-Apr-2001 hojarasca
11/B/BV/113 ALAS 21-Apr-2001 suelo suspendido raices
11/B/AS/114 ALAS 21-Apr-2001 suelo
11/B/BE/115 ALAS 21-Apr-2001 epifitas
11/B/AM/116 ALAS 21-Apr-2001 helecho
11/B/BV/117 ALAS 21-Apr-2001 musgo arbol vivo
11/B/BE/118 ALAS 21-Apr-2001 epifitas
11/B/AS/119 ALAS 21-Apr-2001 suelo
11/B/AS/120 ALAS 21-Apr-2001 suelo
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